We have used targeted mass spectrometry–multiple reaction monitoring (MRM)–to detect the Abl biosensor and its phosphorylated form in relatively small numbers of cells. This read-out strategy enabled the scale-down of the assay, while maintaining the principle of multiplexability.
This project was funded by the NCI Innovative Molecular Analysis Technologies (IMAT) program through R21CA160129, and by the Indiana Clinical Translational Sciences Institute (ICTSI, 5TL1TR001107).